aurka rabbit polyclonal antibody  (Proteintech)

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: Oligonucleotide primers used in the present study.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques: Sequencing

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: In total, 59 control samples and 504 cancer samples were derived from TCGA database for verification. From the 10 genes selected from the two modules, a total of 20 genes were verified. A total of 19 of these changes were verified, and the other one (CXCR4) was excluded. (A) The expression of AURKA, BUB1, CCNB1, CDC45, CDK1, MELK, NUSAP1, PBK, TOP2A and TTK genes in normal lung tissues and lung adenocarcinoma tissues, where red indicates normal lung tissues and green indicates lung adenocarcinoma tissues. (B) The expression of BDKRB2, CCL19, CX3CR1, CXCL13, CXCL9, CXCR4, CXCR5, GNAI1, GNG11 and NMUR1 genes in normal lung tissues and lung adenocarcinoma tissues, with red indicating normal lung tissues and green indicating lung adenocarcinoma tissues. t-test of normal lung tissue and lung adenocarcinoma tissue. *P<0.05, **P<0.01 and ****P<0.0001.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques: Control, Derivative Assay, Expressing

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: ROC curve analysis of 20 genes. AUCs >0.9 were included in the subsequent analysis. A total of 13 genes ( AURKA, CDC45, TTK, TOP2A, CCNB1, NUSAP1, MELK, PBK, BUB1, CDK1, CXCL13, GNG11 and NMUR1 ) were included, and seven genes were excluded. (A) ROC curve analysis was performed for the AURKA, CDC45, TTK, TOP2A and CCNB1 genes sequentially. (B) ROC curve analysis was performed for the NUSAP1, MELK, PBK, BPKPB2 and BUB1 genes sequentially. (C) ROC curve analysis was performed for the CDK1, CCL19, CXCR5, CXCL13 and CXCL9 genes sequentially. (D) ROC curve analysis was performed for the CXCR4, GNG11, GNAL1 and NMUR1, CX3CR1 genes sequentially. ROC, receiver operating characteristic; AUC, area under the curve.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques:

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: Survival analysis was performed and survival curves were obtained using GEPIA database. According to the P-values, there were eight genes with P<0.05 ( AURKA, TOP2A, CCNB1, NUSAP1, MELK, PBK, BUB1 and CDK1). (A-H) The survival curves of CDK1, BUB1, CCNB1, TOP2A, PBK, NUSAP1, AURKA and MELK genes in TCGA database are presented in order. TCGA, The Cancer Genome Atlas.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques:

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: mRNA expression of eight genes screened using WGCNA in clinical tissue samples. Clinical samples are divided into lung adenocarcinoma adjacent tissues and lung adenocarcinoma tissue samples. (A-H) In each graph, the left bar represents the lung adenocarcinoma adjacent tissues, and the right bar the lung adenocarcinoma tissues. The mRNA expression levels of AURKA, BUB1, CCNB1, CDK1, MELK, NUSAP1 and TOP2A genes in lung adenocarcinoma were higher than those in paired adjacent normal tissues. WGCNA, weighted gene co-expression network analysis.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques: Expressing

Journal: Oncology Letters

Article Title: AURKA, TOP2A and MELK are the key genes identified by WGCNA for the pathogenesis of lung adenocarcinoma

doi: 10.3892/ol.2023.13824

Figure Lengend Snippet: Protein expression of seven genes screened using WGCNA in clinical tissue samples. (A) Representative western blots of AURKA, BUB1, CCNB1, CDK1, MELK, NUSAP1 and TOP2A protein expression in adjacent lung adenocarcinoma tissues and lung adenocarcinoma tissue samples. (B) The quantitative analysis of the data in panel A, in which the protein levels of AURKA, TOP2A and MELK in lung adenocarcinoma tissues were higher than those in matched adjacent lung adenocarcinoma tissues. WGCNA, weighted gene co-expression network analysis.

Article Snippet: The antibodies used are as follows: A rabbit anti-AURKA polyclonal antibody (cat. no. A15728), a rabbit anti-BUB1 mitotic checkpoint serine/threonine kinase (BUB1) polyclonal antibody (cat. no. A1929), a rabbit anti-CCNB1 polyclonal antibody (cat. no. A16800), a rabbit anti-CDK1 polyclonal antibody (cat. no. A0220), a rabbit anti-MELK monoclonal antibody (cat. no. A3530), a rabbit anti-nucleolar and spindle associated protein 1 (NUSAP1) polyclonal antibody (cat. no. A16000), a rabbit anti-TOP2A polyclonal antibody (cat. no. A16440) and a mouse monoclonal antibody for β-actin (cat. no. AC004) (all from ABclonal Biotech Co., Ltd. and all at 1:1,000).

Techniques: Expressing, Western Blot